seed storage methods

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Dan Borman
seed storage methods

Hello everyone,

I would like to re-open the seed storage methods discussion. Over the years I have found several interesting sources of information. Gomez-Campo; KEW/FAO; CALGLY

I am still working on passive refrigeration. Please let me know sources of design info on this last, especially in the context of cooling structures as large as 8 feet x 8 feet x 20 feet. This how large we want our seed bank storage to be.

1. Dr. Gomez-Campo http://tinyurl.com/qdulmdp

Dr. Gomez-Campo worked to conserve wild Brassica in seed bank conditions. He tested many different methods of storage. He wrote articles and web pages that clear up many of the topics that I hear being talked about concerning seed storage. The original web page appears to out of commission. I used Google cache to access. Lots of info there in associated pages. Again use the cache function...

http://tinyurl.com/ou2ddx4

or use Wayback machine:

http://tinyurl.com/mhgaj9p

http://www.bancodegermoplasma.upm.es/documentos/P%C3%A9rez%20Garc%C3%ADa...

2. KEW/FAO

http://tinyurl.com/m8d44jg

3. CALGLY is the most interesting to me as I would like to tend toward a seed storage method that either uses no refrigeration or totally passive refrigeration; or a combination.

The following is copied from ECHO's website:

EDN
ECHO DEVELOPMENT NOTES
17391
Durrance Road, North Fort Myers, FL 33917-2239
Phone: (239) 543-3246, Fax: (239) 543-
5317 USA
E-mail:
echo@echonet.org
, Web site:
http://www.echonet.org
ISSUE 57
JULY
1997
EDITED BY MARTIN PRICE
A NEW WAY TO PRESERVE VIABILITY OF SEED
IN REMOTE LOCATIONS.

By Martin Price.

Saving seed is a problem for farmers in hot, humid climates. It is even more of a problem for community, mission, or government seedbanks that must preserve seed for long timeperiods in remote locations without refrigeration. We believe the work of Dr. D. K. Pandey in India may be a breakthrough in reliable seed preservation in the rural tropics. He published an article in Scientia Horticulturae 66 (1996, pp. 1-8) entitled "A suitable liquid preservative for enhancing longevity of orthodox seeds." He calls this special seed-preserving liquid CALGLY, named after the two relatively common chemicals that are used to make it: calcium chloride (CaCl2) and glycerol. We were easily able to locate both substances for sale in Ft. Myers. CaCl2 sells for US$9.50 per gallon (sold as flakes), while glycerol costs US$45.45 per gallon. Initial experiments had been done with pure glycerol, but seed absorbed the glycerol and was killed. Dr. Pandey discovered that adding CaCl2 to the glycerol prevented the liquid from being absorbed by seed. CALGLY is made by heating glycerol to 156-160°C and then adding CaCl2, which dissolves. For every 75 grams of glycerol 25 g of CaCl2 is added.

Onion Seed Experiment.
Onion seed is known for its tendency to lose viability during storage. Dr. Pandey dried fresh onion seed in the sun and found that this method reduced the moisture content (MC) of the seed to 10.5%. In his experiment, he used laboratory techniques to dry samples of onion seed to 10%, 8% and 2% MC.
It is well known that reducing the MC of seed even a few percentage points below what is possible to obtain by drying in the sun can improve seed storage life. Samples of seed at 10%, 8%, and 2% MC were stored in laminated aluminum foil seed packets (probably similar to the packaging in which
ECHO's seed is sent) while samples of 10% and 2%MC from the same lots were stored in vials to which CALGLY was added. The volume of CALGLY added to the vials was the same as the volume of the seed.
The packages and vials were stored for various lengths of time under natural temperatures which were typical of tropical conditions. Temperatures ranged between 15 and 45°C (59 to 113°F).

After 300 days, the viability of the seed stored in foil packages at 10% MC had dropped from an initial 96% to 0%. The seed lot stored at 8% MC had a remarkable 89% viability after 900 days. However, in 1100 days it had dropped to 0%. The seed that had been reduced in the laboratory to 2% MC still had a 90% viability after 2190 days (six years) when sealed in the laminated foil packet.
How did the seed stored in CALGLY fare? The seed with 10% MC did just as well as the non-CALGLY seed which had been reduced to 2% MC--both had 93% viability after 2190 days.

How about other kinds of seed? Seed of four other vegetables were evaluated: capsicum (Capsicum annumL. var. 'Arka Gauraw'), chili (Capsicum annumL. var. 'Arka Lohit'), carrot (Daucus carotaL. var. 'Nantes') and pea(Pisum sativumL. var. 'HUP'). The four seedlots at the start of the experiment had MC readings of 8.4, 7.78, 8.3 and 9.0% repectively. The seedlots in foil packets became non-viable after 2, 4, 4, and 3 years. None of the seed immersed in CALGLY showed any significant loss of viability even after six years of ageing.

How does it work? Dr. Pandey believes the CALGLY solution works by "ultradrying" seed. He bases this on the fact that the performance in CALGLY was similar to that of seed in foil packets which had been ultradried using "time-consuming, cumbersome and expensive techniques." However, at the end of the experiment he could not measure the precise MC of seed stored in CALGY because the viscous material coated the small seed and could not be removed sufficiently for the precise weighing that would be necessary. He has experiments underway to see if other seed can likewise be preserved in CALGLY. At ECHO we intend to do some tests of our own.

Is CALGLY dangerous? Both glycerol and CaCl2 are relatively non-toxic, are not mutagens, do not harm seed or plants, and do not pose corrosion, fire, health or environmental hazards. Glycerol is a constituent of antifreeze used in automobiles. Because of its sweet taste (about half the sweetness of cane sugar), you should not leave a large amount where an animal might be tempted to drink it.

Calcium chloride is used to melt ice on sidewalks, to fireproof fabrics, in antifreeze mixtures, in fire extinguishers and in dust control on unpaved roads.

 

 

wabonsall
Thanks so much for that input

Thanks so much for that input, Dan. I agree that the topic must be elaborated on with continued discussion. For my part i"m researching  economical technologies for long-term  seed storage, which would make my own Scatterseed Project vastly more cost-effective. For now i'm looking at solar-powered freezers, quite effective and more secure than the grid certainly, but still not without hitched. This passive refrigeration bears looking into. It helps that we do not necessarily require below-freezing temps and that power interruption, though a problem, is not fatal (as with fresh-frozen food.